Journal: Pathogens

Article Title: Rapid Detection of Quinolone Resistance Mutations in gyrA of Helicobacter pylori by Real-Time PCR

doi: 10.3390/pathogens11010059

Figure Lengend Snippet: Sequence of wild-type and mutant codons of gyrA QRDR.

Article Snippet: The WT1 gyrA sequence was amplified by PCR with the FP and RP GyrA WT1 primers ( ) using sequenced patient DNA as a template, and cloned into the vector pUC57 via the restriction sites XbaI and BamHI (Genscript, Piscataway, NJ, USA).

Techniques: Sequencing, Mutagenesis

Journal: Pathogens

Article Title: Rapid Detection of Quinolone Resistance Mutations in gyrA of Helicobacter pylori by Real-Time PCR

doi: 10.3390/pathogens11010059

Figure Lengend Snippet: Cloning and mutagenesis primers.

Article Snippet: The WT1 gyrA sequence was amplified by PCR with the FP and RP GyrA WT1 primers ( ) using sequenced patient DNA as a template, and cloned into the vector pUC57 via the restriction sites XbaI and BamHI (Genscript, Piscataway, NJ, USA).

Techniques: Cloning, Mutagenesis, Sequencing

Journal: Pathogens

Article Title: Rapid Detection of Quinolone Resistance Mutations in gyrA of Helicobacter pylori by Real-Time PCR

doi: 10.3390/pathogens11010059

Figure Lengend Snippet: Sequences of hybridization probes and primers used for RT-PCR.

Article Snippet: The WT1 gyrA sequence was amplified by PCR with the FP and RP GyrA WT1 primers ( ) using sequenced patient DNA as a template, and cloned into the vector pUC57 via the restriction sites XbaI and BamHI (Genscript, Piscataway, NJ, USA).

Techniques: Hybridization, Sequencing

Journal: Pathogens

Article Title: Rapid Detection of Quinolone Resistance Mutations in gyrA of Helicobacter pylori by Real-Time PCR

doi: 10.3390/pathogens11010059

Figure Lengend Snippet: T m -values of MT1 and MT3 probes.

Article Snippet: The WT1 gyrA sequence was amplified by PCR with the FP and RP GyrA WT1 primers ( ) using sequenced patient DNA as a template, and cloned into the vector pUC57 via the restriction sites XbaI and BamHI (Genscript, Piscataway, NJ, USA).

Techniques:

Journal: Pathogens

Article Title: Rapid Detection of Quinolone Resistance Mutations in gyrA of Helicobacter pylori by Real-Time PCR

doi: 10.3390/pathogens11010059

Figure Lengend Snippet: Assignment of patient DNA samples by melting curve analyses and DNA sequencing.

Article Snippet: The WT1 gyrA sequence was amplified by PCR with the FP and RP GyrA WT1 primers ( ) using sequenced patient DNA as a template, and cloned into the vector pUC57 via the restriction sites XbaI and BamHI (Genscript, Piscataway, NJ, USA).

Techniques: Sequencing, DNA Sequencing

Journal: Pathogens

Article Title: Rapid Detection of Quinolone Resistance Mutations in gyrA of Helicobacter pylori by Real-Time PCR

doi: 10.3390/pathogens11010059

Figure Lengend Snippet: Sequencing data obtained from DNA in gastric biopsies of H. pylori -positive patients. Eight DNA samples ( A – H ) used for melting curve analysis were sequenced with the gyrA -RP as sequencing primer . The relevant region of the QRDR harboring codons 87 and 91 is shown.

Article Snippet: The WT1 gyrA sequence was amplified by PCR with the FP and RP GyrA WT1 primers ( ) using sequenced patient DNA as a template, and cloned into the vector pUC57 via the restriction sites XbaI and BamHI (Genscript, Piscataway, NJ, USA).

Techniques: Sequencing

Journal: BMC Biotechnology

Article Title: Cloning, expression and biochemical characterization of the cholesterol oxidase CgChoA from Chryseobacterium gleum

doi: 10.1186/1472-6750-14-46

Figure Lengend Snippet: Comparison of different aspects concerning the production and selected properties of cholesterol oxidases

Article Snippet: E. coli strain JM109 [genotype endA 1 recA 1, gyrA 96, thi , hsdR 17,(r K - , m K + ), relA 1, supE 44, λ - , Δ (lac-proAB) , (F', traD 36, proAB , lacI q ZΔM15)] and the pQE-30 expression vector were purchased from Promega (Madison, USA) and Qiagen (Valencia, USA), respectively.

Techniques: Comparison, Expressing, Purification, Activity Assay, Modification, Enzymatic Assay, Column Chromatography